Composite

Part:BBa_K1453404:Design

Designed by: Yangyang Li, Binhan Hao, Renhe Luo   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-17)


ssDsbA-Lgt-pykF-TAL1-His Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2347
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2062
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2347
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2347
    Illegal AgeI site found at 862
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 483
    Illegal BsaI.rc site found at 2968
    Illegal BsaI.rc site found at 3580


Design Notes

This part can recognize a special sequence, T-TCGATATCAAGC-T. This part contains the pyruvate kinase (EC:2.7.1.40).


Source

E.coli, BBa_K1453403, BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.

References